In:
FEBS Letters, Wiley, Vol. 347, No. 1 ( 1994-06-20), p. 69-72
Abstract:
In brain and many other tissues, Type I inositol 1,4,5‐trisphosphate (InsP 3 ) 5‐phosphatase is the major isozyme hydrolysing the calcium‐mobilizing second messenger InsP 3 . We recently reported the cloning and expression of dog thyroid InsP 3 5‐phosphatase. During the course of this cloning, screening of a human brain cDNA library allowed us to isolate a cDNA clone D1 with 91% sequence identity with the thyroid sequence. When clone D1 was expressed in Escherichia coli , the fusion protein had InsP 3 5‐phosphatase activity. M r estimates of the recombinant enzyme made by unmunodetection, activity assay after SDS/PAGE or silver staining were consistent with the calculated molecular mass. In situ hybridization on human cerebellum sections localised the mRNA for this enzyme to the Purkinje cells.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/0014-5793(94)00509-5
Language:
English
Publisher:
Wiley
Publication Date:
1994
detail.hit.zdb_id:
1460391-3
SSG:
12
