In:
FEBS Letters, Wiley, Vol. 374, No. 1 ( 1995-10-23), p. 34-38
Abstract:
The systematic isolation of circulating regulatory peptides which generate cGMP as second messenger resulted in the identification of a novel member of the guanylin family. In the present study we describe the purification and amino acid sequence of a new guanylate cyclase C activating peptide (GCAP‐II). GCAP‐II contains 24 amino acids in the following sequence: FKTLRTIANDDCELCVNVACTGCL. Its molecular mass is 2597.7 Da. The 16 C‐terminal amino acids are identical to uroguanylin from human urine. Native and synthetic GCAP‐II activate GC‐C, the specific guanylate cyclase receptor, of cultured human colon carcinoma (T84) cells. GCAP‐II stimulates chloride secretion in isolated human intestinal mucosa mediated by intracellular cGMP increase. GCAP‐II specific antibodies were used to localize the peptide by immunohistochemistry in entero‐endocrine cells of the colonic mucosa.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/0014-5793(95)01075-P
Language:
English
Publisher:
Wiley
Publication Date:
1995
detail.hit.zdb_id:
1460391-3
SSG:
12