In:
FEBS Letters, Wiley, Vol. 470, No. 3 ( 2000-03-31), p. 227-231
Abstract:
We have used the yeast two‐hybrid system to search for cytoplasmic proteins that might assist in the intracellular trafficking of the soluble β‐galactoside‐binding protein, galectin‐3. We utilised as bait murine full‐length galectin‐3 to screen a murine 3T3 cDNA library. Several interacting clones were found to encode a partial open reading frame and a full‐length clone was obtained by rapid amplification of cDNA ends methodology. In various assays in vitro the novel protein was shown to bind galectin‐3 in a carbohydrate‐independent manner. The novel protein contains an unusually high content of cysteine and histidine residues and shows significant sequence homologies with several metal ion‐binding motifs present in known proteins. Confocal immunofluorescence microscopy of permeabilised 3T3 cells shows a prominent perinuclear, as well as cytoplasmic, localisation of the novel protein.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/S0014-5793(00)01310-7
Language:
English
Publisher:
Wiley
Publication Date:
2000
detail.hit.zdb_id:
1460391-3
SSG:
12