In:
FEBS Letters, Wiley, Vol. 475, No. 3 ( 2000-06-23), p. 232-236
Kurzfassung:
Previous studies have suggested that human salivary secretory leukocyte protease inhibitor (SLPI) inhibits HIV‐1 by binding to a host cell surface protein of unknown identity. Using the yeast two‐hybrid assay, we identified a gene sequence encoding a novel SLPI‐binding protein (SLPI‐BP). The 1.5‐kb cDNA encodes a 318‐amino acid protein with a predicted transmembrane segment near the C‐terminus. Sequence analysis revealed that SLPI‐BP is the human scramblase protein that is involved in the movement of membrane phospholipids. Co‐expression of SLPI and SLPI‐BP followed by an S‐protein pulldown assay confirmed the specific interaction between these two proteins. Our data represent the first report for the identity of SLPI‐BP.
Materialart:
Online-Ressource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/S0014-5793(00)01700-2
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2000
ZDB Id:
1460391-3
SSG:
12
