In:
FEBS Letters, Wiley, Vol. 529, No. 2-3 ( 2002-10-09), p. 168-172
Abstract:
Plexins are receptors for the repulsive axon guidance molecules semaphorins. Previously, we have shown that plexin‐B1 binds activated Rac, but that clustering of plexin‐B1 causes Rho activation, resulting in stress fiber formation. Using the yeast two‐hybrid system, we found that the C‐terminus of B plexins interacted directly with Rho‐specific exchange factors, via their PDZ domain. Mutation of the carboxy‐terminal amino acids of plexin‐B1 or coexpression of a dominant negative PDZ‐RhoGEF abrogated the ability of plexin‐B1 to cause stress fiber formation. Our results demonstrate a role for PDZ‐RhoGEF in B plexin‐mediated activation of Rho/Rho kinase signaling, implicated in the regulation of axon guidance and cell migration.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/S0014-5793(02)03323-9
Language:
English
Publisher:
Wiley
Publication Date:
2002
detail.hit.zdb_id:
1460391-3
SSG:
12