In:
FEBS Letters, Wiley, Vol. 408, No. 1 ( 1997-05-12), p. 99-104
Abstract:
Some of the numerous functions of the growth suppressor protein p53 are regulated by its interaction with viral and cellular proteins. C‐terminal sequences of p53 are implicated in binding to the regulatory β‐subunit of protein kinase CK2. Using a p53‐specific DNA binding element we found that the β‐subunit of CK2 inhibited the DNA binding of p53 whereas the α‐subunit had no influence. The CK2 holoenzyme consisting of two α‐ and two β‐subunits led to a supershift in DNA binding of p53 similar to the p53‐specific monoclonal antibody PAb421 as well as the C‐terminus of p53. Thus, our results showed an individual role of the free β‐subunit of CK2 on the DNA binding activity of p53.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/S0014-5793(97)00399-2
Language:
English
Publisher:
Wiley
Publication Date:
1997
detail.hit.zdb_id:
1460391-3
SSG:
12