In:
FEBS Letters, Wiley, Vol. 441, No. 3 ( 1998-12-28), p. 407-412
Abstract:
The nucleotide sequence of the monoclonal antibody F124, specific for the preS2 region of the surface antigen of hepatitis B virus, has been determined and an single‐chain Fv fragment (scFv) recombinant construction has been cloned and expressed into the periplasmic region of Escherichia coli . The recombinant antibody fragment contains a (Gly 4 Ser) 3 linker connecting the C‐terminus of the heavy chain variable region (V H ) domain to the N‐terminus of the light chain variable region (V L ) domain. A 23‐residue peptide segment, containing a c‐ myc marker for immunochemical detection of the scFv and hexahistidine tag to facilitate its purification, was added C‐terminal to the V L domain. The scFv mimics the antibody in binding to the native antigen in the form of recombinant hepatitis B surface antigen (HBsAg) particles as well as to peptide fragments carrying the viral epitope.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/S0014-5793(98)01581-6
Language:
English
Publisher:
Wiley
Publication Date:
1998
detail.hit.zdb_id:
1460391-3
SSG:
12