In:
British Journal of Nutrition, Cambridge University Press (CUP), Vol. 105, No. 9 ( 2011-05-14), p. 1329-1336
Abstract:
The activation of PPARγ by ligands, including conjugated linoleic acid (CLA) isomers, plays an important role in the immune response. Among CLA isomers, trans -10, cis -12 ( t 10 c 12)-CLA is known to participate in the modulation of pro-inflammatory cytokine secretion. The aim of the present study was to assess the effect of t 10 c 12-CLA on PPARγ activation, NF-κB activation and TNF-α expression in lipopolysaccharide (LPS)-naive and LPS-stimulated porcine peripheral blood mononuclear cells (PBMC). In addition, the effect of PPARγ inhibition on NF-κB activation and TNF-α expression in porcine PBMC was examined. t 10 c 12-CLA was found to increase TNF-α expression and NF-κB activity in LPS-naive porcine PBMC. In contrast, t 10 c 12-CLA decreased TNF-α expression and NF-κB activity in LPS-stimulated porcine PBMC. t 10 c 12-CLA up-regulated PPARγ activity and mRNA expression in both LPS-naive and LPS-stimulated porcine PBMC. GW9662, a PPARγ antagonist, completely negated the modulating effects of t 10 c 12-CLA on TNF-α expression and NF-κB activity in both LPS-naive and LPS-stimulated porcine PBMC. These results suggest that t 10 c 12-CLA can modulate TNF-α production and NF-κB activation by a PPARγ-dependent pathway in porcine PBMC.
Type of Medium:
Online Resource
ISSN:
0007-1145
,
1475-2662
DOI:
10.1017/S000711451000499X
Language:
English
Publisher:
Cambridge University Press (CUP)
Publication Date:
2011
detail.hit.zdb_id:
2016047-1
SSG:
12
SSG:
21