In:
British Journal of Nutrition, Cambridge University Press (CUP), Vol. 109, No. 6 ( 2013-03-28), p. 990-1000
Abstract:
Fish oil supplementation during pregnancy has been associated with lower levels of cord blood IL-13, suggesting that the administration of n -3 fatty acids may attenuate the development of allergic disease. The present study aimed to investigate the mechanism by which n -3 fatty acid administration influences the production of IL-13. Pregnant BALB/c mice were fed nutritionally complete high-fat diets (15 %, w/w) with an n -3 fatty acid-enriched (DHA 1 %, w/w) or control diet (0 % DHA) immediately following delivery. Pups were exposed during suckling and weaned to the maternal diet for the remainder of the study. The production of IL-13, IL-4, IL-10 and interferon-γ from the splenocytes of ovalbumin (ova)-sensitised animals was assessed following in vitro ova stimulation or unstimulated conditions. Human T helper type 2 (Th2) cells were mitogen-stimulated in the presence or absence of DHA (10 μ m ) and assessed for IL-13 and IL-4 expression using intracellular flow cytometry. The influence on transcriptional activation was studied using a human IL-13 promoter reporter construct and electromobility shift assay. Ova-activated splenocytes from DHA-fed mice produced less IL-13 (57·2 ( se 21·7) pg/ml) and IL-4 (7·33 ( se 3·4) pg/ml) compared with cells from the animals fed the control diet (161·5 ( se 45·0), P 〈 0·05; 33·2 ( se 11·8), P 〈 0·05). In vitro , DHA inhibited the expression of IL-13 protein from human Th2 cells as well as transcriptional activation and binding of the transcription factors cyclic AMP response element binding and activating transcription factor 2 to the human IL-13 promoter. These data indicate the potential of n -3 fatty acids to attenuate IL-13 expression, and suggest that they may subsequently reduce allergic sensitisation and the development of allergic disease.
Type of Medium:
Online Resource
ISSN:
0007-1145
,
1475-2662
DOI:
10.1017/S0007114512002917
Language:
English
Publisher:
Cambridge University Press (CUP)
Publication Date:
2013
detail.hit.zdb_id:
2016047-1
SSG:
12
SSG:
21
