In:
British Journal of Nutrition, Cambridge University Press (CUP), Vol. 127, No. 3 ( 2022-02-14), p. 384-397
Kurzfassung:
Non-resolving inflammation is characteristic of tuberculosis (TB). Given their inflammation-resolving properties, n -3 long-chain PUFA ( n -3 LCPUFA) may support TB treatment. This research aimed to investigate the effects of n -3 LCPUFA on clinical and inflammatory outcomes of Mycobacterium tuberculosis -infected C3HeB/FeJ mice with either normal or low n -3 PUFA status before infection. Using a two-by-two design, uninfected mice were conditioned on either an n -3 PUFA-sufficient ( n -3FAS) or -deficient ( n -3FAD) diet for 6 weeks. One week post-infection, mice were randomised to either n -3 LCPUFA supplemented ( n -3FAS/ n -3+ and n -3FAD/n-3+) or continued on n -3FAS or n -3FAD diets for 3 weeks. Mice were euthanised and fatty acid status, lung bacterial load and pathology, cytokine, lipid mediator and immune cell phenotype analysed. n -3 LCPUFA supplementation in n -3FAS mice lowered lung bacterial loads ( P = 0·003), T cells ( P = 0·019), CD4 + T cells ( P = 0·014) and interferon (IFN)- γ ( P 〈 0·001) and promoted a pro-resolving lung lipid mediator profile. Compared with n -3FAS mice, the n -3FAD group had lower bacterial loads ( P = 0·037), significantly higher immune cell recruitment and a more pro-inflammatory lipid mediator profile, however, significantly lower lung IFN- γ , IL-1 α , IL-1 β and IL-17, and supplementation in the n -3FAD group provided no beneficial effect on lung bacterial load or inflammation. Our study provides the first evidence that n -3 LCPUFA supplementation has antibacterial and inflammation-resolving benefits in TB when provided 1 week after infection in the context of a sufficient n -3 PUFA status, whilst a low n -3 PUFA status may promote better bacterial control and lower lung inflammation not benefiting from n -3 LCPUFA supplementation.
Materialart:
Online-Ressource
ISSN:
0007-1145
,
1475-2662
DOI:
10.1017/S0007114521001124
Sprache:
Englisch
Verlag:
Cambridge University Press (CUP)
Publikationsdatum:
2022
ZDB Id:
2016047-1
SSG:
12
SSG:
21
