In:
Microscopy and Microanalysis, Oxford University Press (OUP), Vol. 4, No. S2 ( 1998-07), p. 444-445
Kurzfassung:
Microscope specimens are 3-dimensional objects, but the images from conventional light microscopes are flat - they only show two dimensions. Multiple oblique illumination is a novel lighting technique for transmitted light microscopes that produces multiple (i.e. two or more) high definition 3-dimensional images using conventional microscope objective lenses. We have previously described its use in transmitted light. The same optical theory has now been expanded to include reflection microscopy. In the present paper, we describe a new development which will make this approach more widely available. It is a retrofit illumination system that will produce true 3-dimensional images directly through the eyepieces of conventional microscopes. By seeing z-axis information in real-time and in the context of a specimen's entire thickness, researchers can gain additional, unambiguous information about the interrelationships between structures, whereas critical information about 3-dimensional structures can be obscured, lost or misinterpreted when using 2-dimensional instruments. The importance of accurate z-axis information has popularized methods such as deconvolution and confocal microscopy.
Materialart:
Online-Ressource
ISSN:
1431-9276
,
1435-8115
DOI:
10.1017/S1431927600022340
Sprache:
Englisch
Verlag:
Oxford University Press (OUP)
Publikationsdatum:
1998
ZDB Id:
1481716-0
SSG:
11
SSG:
12
