In:
Chinese Journal of Agricultural Biotechnology, Cambridge University Press (CUP), Vol. 6, No. 1 ( 2009-04), p. 49-53
Abstract:
An efficient AHL ( N -acyl-homoserine lactone) bioassay strain, JZA1, of Agrobacterium tumefaciens was used to detect the AHL production from Acidovorax avenae subsp. citrulli [the pathogen causing bacterial fruit blotch (BFB) of melons], and the results showed that A. avenae subsp. citrulli produced a 3-O-C 8 -homoserine (HSL) type signal molecule. Gene aiiA , which could degrade AHL molecules, was transformed into A. avenae subsp. citrulli strain NJF10, creating strain NJF10- aiiA . The AHL production from NJF10- aiiA was significantly reduced compared with wild-type NJF10. Inoculation tests showed that NJF10- aiiA had an obvious reduction of virulence on watermelon fruits. Our finds showed that AHL production by A. avenae subsp. citrulli was related to its pathogenicity. This work might provide a novel way to control BFB by QS (quorum sensing) interference.
Type of Medium:
Online Resource
ISSN:
1479-2362
,
1479-2370
DOI:
10.1017/S1479236209002514
Language:
English
Publisher:
Cambridge University Press (CUP)
Publication Date:
2009