In:
Allergy, Wiley, Vol. 54, No. 7 ( 1999-07), p. 742-747
Abstract:
Background: The major allergenic component of bee venom is phospholipase A 2 (PLA2). Methods: In this study, PLA2 was used to analyze and enrich PLA2‐binding cells from peripheral blood by high gradient magnetic cell sorting. Results: In normal donors, the frequency of allergen (PLA2)‐binding cells among peripheral blood mononuclear cells (PBMC) as determined by flow cytometry is below 0.1%, whereas in bee‐venom‐allergic patients, PLA2‐binding cells are readily detectable at frequencies of up to 2.3%. In severely bee‐venom‐allergic patients, many basophilic granulocytes are present, as defined by anti‐CD9, CD25, and CD38 mAb, comprising up to 95% of the PLA2‐binding cells. From blood of allergic and normal donors, about equal absolute numbers of allergen‐binding CD19/21‐positive B cells can be enriched. Severe anaphylactic reactions (Mueller grade IV) and failure of or adverse reactions during immunotherapy are associated with high numbers of circulating allergen‐binding basophils. Interestingly, in the patients studied, the number of PLA2‐binding basophilic granulocytes did not markedly change during rush immunotherapy and up to 6 months of maintenance immunotherapy. Conclusions: The specific and reproducible enrichment of PLA2‐binding cells provides a new tool for the analysis and monitoring of effector cells in bee‐venom‐allergic patients with immediate‐type hypersensitivity.
Type of Medium:
Online Resource
ISSN:
0105-4538
,
1398-9995
DOI:
10.1034/j.1398-9995.1999.00952.x
Language:
English
Publisher:
Wiley
Publication Date:
1999
detail.hit.zdb_id:
2003114-2
