In:
APMIS, Wiley, Vol. 110, No. 7-8 ( 2002-08), p. 545-553
Abstract:
The p 53 gene, a tumour suppressor gene located on the short arm of chromosome 17 (17pl3), is frequently mutated in various human tumours. Accumulation of p 53 protein in neoplastic cells and its relea se following tumour necrosis can lead to development of circulating autoantibodies (AAb) against p 53. Earlier studies of ovarian cancer (OC) patients reported different frequencies of p 53 AAb and conclusions regarding the clinical and prognostic value of these AAb have not been in agreement. We therefore analysed for the presence of p 53 AAb in a total of 227 preoperative serum samples from 193 OC patients and 34 patients with ovarian borderline tumours, and, in addition, serum samples from 86 healthy controls. An enzyme‐linked immunosorbent assay (ELISA) was used to measure serum IgG antibodies against p53. The p 53 protein used in the assay was produced as a hexahistidine‐tagged fusion protein by baculovirus‐infected Spodoptera frugiperda cells. Cut‐off values for p 53 AAb were evaluated, and correlations of p 53 AAb with clinical‐, biochemical data and survival were examined. We found a low sensitivity for p 53 AAb alone, and no major additional effect of the detection rate of CA125 was found. No significant associations were found between p 53 AAb and clinical stage, age, histological subtype and radicality after primary surgery. In contrast, we found significantly elevated CA125 levels in p 53 AAb‐positive patients compared to lower CA125 levels in p 53 AAb‐negative patients ( p =0.003). No significant differences were found between p 53 AAb‐positive and p 53 AAb‐negative patients in the univariate and multivariate survival analyses. In conclusion, in a screening study for OC serum p 53 AAb levels are of no diagnostic value, even if combined with the tumour marker CA125. The presence of increased serum p 53 AAb in patients with diagnosed OC could not be correlated with any clinical data and preoperative serum p 53 AAb status had no evident value.
Type of Medium:
Online Resource
ISSN:
0903-4641
,
1600-0463
DOI:
10.1034/j.1600-0463.2002.11007805.x
Language:
English
Publisher:
Wiley
Publication Date:
2002
detail.hit.zdb_id:
2098213-6
SSG:
12
