In:
Experimental & Molecular Medicine, Springer Science and Business Media LLC, Vol. 54, No. 11 ( 2022-11-28), p. 2077-2091
Abstract:
Necroptosis is the major cause of death in alveolar epithelial cells (AECs) during acute lung injury (ALI). Here, we report a previously unrecognized mechanism for necroptosis. We found an accumulation of mitochondrial citrate (citrate mt ) in lipopolysaccharide (LPS)-treated AECs because of the downregulation of Idh3α and citrate carrier (CIC, also known as Slc25a1). shRNA- or inhibitor–mediated inhibition of Idh3α and Slc25a1 induced citrate mt accumulation and necroptosis in vitro. Mice with AEC-specific Idh3α and Slc25a1 deficiency exhibited exacerbated lung injury and AEC necroptosis. Interestingly, the overexpression of Idh3α and Slc25a1 decreased citrate mt levels and rescued AECs from necroptosis. Mechanistically, citrate mt accumulation induced mitochondrial fission and excessive mitophagy in AECs. Furthermore, citrate mt directly interacted with FUN14 domain-containing protein 1 (FUNDC1) and promoted the interaction of FUNDC1 with dynamin-related protein 1 (DRP1), leading to excessive mitophagy-mediated necroptosis and thereby initiating and promoting ALI. Importantly, necroptosis induced by citrate mt accumulation was inhibited in FUNDC1-knockout AECs. We show that citrate mt accumulation is a novel target for protection against ALI involving necroptosis.
Type of Medium:
Online Resource
ISSN:
2092-6413
DOI:
10.1038/s12276-022-00889-8
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2022
detail.hit.zdb_id:
2084833-X