In:
Cell Death & Disease, Springer Science and Business Media LLC, Vol. 10, No. 2 ( 2019-01-18)
Abstract:
NF-κB functions as modulator of T cell receptor-mediated signaling and transcriptional regulator of miR-34a. Our in silico analysis revealed that miR-34a impacts the NF-κB signalosome with miR-34a binding sites in 14 key members of the NF-κB signaling pathway. Functional analysis identified five target genes of miR-34a including PLCG1 , CD3E , PIK3CB , TAB2 , and NFΚBIA . Overexpression of miR-34a in CD4 + and CD8 + T cells led to a significant decrease of NFΚBIA as the most downstream cytoplasmic NF-κB member, a reduced cell surface abundance of TCRA and CD3E, and to a reduction of T cell killing capacity. Inhibition of miR-34a caused an increase of NFΚBIA, TCRA, and CD3E. Notably, activation of CD4 + and CD8 + T cells entrails a gradual increase of miR-34a. Our results lend further support to a model with miR-34a as a central NF-κB regulator in T cells.
Type of Medium:
Online Resource
ISSN:
2041-4889
DOI:
10.1038/s41419-018-1295-1
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2019
detail.hit.zdb_id:
2541626-1
