In:
Nature Communications, Springer Science and Business Media LLC, Vol. 9, No. 1 ( 2018-06-08)
Kurzfassung:
CRISPR advances genome engineering by directing endonuclease sequence specificity with a guide RNA molecule (gRNA). For precisely targeting a gene for modification, each genetic construct requires a unique gRNA. By generating a gRNA against the flippase recognition target (FRT) site, a common genetic element shared by multiple genetic collections, CRISPR-FRT circumvents this design constraint to provide a broad platform for fast, scarless, off-the-shelf genome engineering.
Materialart:
Online-Ressource
ISSN:
2041-1723
DOI:
10.1038/s41467-018-04651-5
Sprache:
Englisch
Verlag:
Springer Science and Business Media LLC
Publikationsdatum:
2018
ZDB Id:
2553671-0
