In:
Nature Communications, Springer Science and Business Media LLC, Vol. 12, No. 1 ( 2021-06-29)
Abstract:
PrimPol is a human DNA polymerase-primase that localizes to mitochondria and nucleus and bypasses the major oxidative lesion 7,8-dihydro-8-oxoguanine (oxoG) via translesion synthesis, in mostly error-free manner. We present structures of PrimPol insertion complexes with a DNA template-primer and correct dCTP or erroneous dATP opposite the lesion, as well as extension complexes with C or A as a 3′−terminal primer base. We show that during the insertion of C and extension from it, the active site is unperturbed, reflecting the readiness of PrimPol to accommodate oxoG (anti) . The misinsertion of A opposite oxoG (syn) also does not alter the active site, and is likely less favorable due to lower thermodynamic stability of the oxoG (syn) •A base - pair. During the extension step, oxoG (syn) induces an opening of its base-pair with A or misalignment of the 3′-A primer terminus. Together, the structures show how PrimPol accurately synthesizes DNA opposite oxidatively damaged DNA in human cells.
Type of Medium:
Online Resource
ISSN:
2041-1723
DOI:
10.1038/s41467-021-24317-z
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2021
detail.hit.zdb_id:
2553671-0
