In:
Scientific Reports, Springer Science and Business Media LLC, Vol. 7, No. 1 ( 2017-06-30)
Abstract:
Artemisinins are the current mainstay of malaria chemotherapy. Their exact mode of action is an ongoing matter of debate, and several factors have recently been reported to affect an early stage of artemisinin resistance of the most important human malaria parasite Plasmodium falciparum . Here, we identified a locus on chromosome 7 that affects the artemisinin susceptibility of P. falciparum in a quantitative trait locus analysis of a genetic cross between strains 7G8 and GB4. This locus includes the peroxiredoxin gene PFAOP . However, steady-state kinetic data with recombinant Pf AOP do not support a direct interaction between this peroxidase and the endoperoxide artemisinin. Furthermore, neither the overexpression nor the deletion of the encoding gene affected the IC 50 values for artemisinin or the oxidants diamide and tert -butyl hydroperoxide. Thus, Pf AOP is dispensable for blood stage parasite survival, and the correlation between the artemisinin susceptibility and chromosome 7 is probably based on another gene within the identified locus.
Type of Medium:
Online Resource
ISSN:
2045-2322
DOI:
10.1038/s41598-017-04277-5
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2017
detail.hit.zdb_id:
2615211-3
