In:
Scientific Reports, Springer Science and Business Media LLC, Vol. 10, No. 1 ( 2020-03-20)
Abstract:
Splenic immune function was enhanced in diet-induced-obese (DIO) mice caused by Escherichia coli . The changes in spleen function on apoptosis were still unknown. Two hundred mice in groups Lean- E. coli and DIO- E. coli were intranasal instillation of E. coli . And another two hundred mice in groups Lean-PBS and DIO-PBS were given phosphate-buffered saline (PBS). Subsequently, spleen histology was analyzed. Then the rates of spleen cell (SC) apoptosis, and expression of the genes and proteins of Bcl-2, Bax, caspase-3 and caspase-9 were quantified in each group at 0 h (uninfected), 12 h, 24 h, and 72 h postinfection. The SC apoptosis rates of the DIO- E. coli groups were lower than those of the DIO-PBS groups at 12, 24 and 72 h ( p 〈 0.05). Anti-apoptotic Bcl-2 expression gene and protein of the DIO- E. coli groups were higher than those of the DIO-PBS groups ( p 〈 0.05). Gene expressions of pro-apoptotic Bax, caspase-3 and caspase-9 of the DIO- E. coli groups were lower than those of DIO-PBS groups at 12, 24 and 72 h ( p 〈 0.05). The SC apo p tosis rates of the Lean- E. coli groups were higher than those of the Lean- PBS groups at 12 h and 24 h ( p 〈 0.05). Interestingly, the SC a p optosis rates in the DIO- E. coli groups were lower than those of the Lean- E. coli groups at 12 h ( p 〈 0.05). In conclusion, our results suggested that the DIO mice presented stronger anti-apoptotic abilities than Lean mice in non-fatal acute pneumonia induced by E. coli infection, which is more conducive to protecting the spleen and improving the immune defense ability of the body.
Type of Medium:
Online Resource
ISSN:
2045-2322
DOI:
10.1038/s41598-020-62044-5
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2020
detail.hit.zdb_id:
2615211-3
