In:
Scientific Reports, Springer Science and Business Media LLC, Vol. 11, No. 1 ( 2021-07-28)
Abstract:
The role of the long noncoding RNA CCAT1 NC_000008.10:g.128220661C 〉 T (rs67085638) in the development of colon cancer has been reported. Therefore, we assessed the prevalence of rs67085638 in patients with gastric cancer (GC). We also evaluated the effect of rs67085638 on B-cell-specific Moloney leukaemia virus insertion site 1 (BMI1) transcripts in primary GC and counterpart histopathologically confirmed disease-free margin tissue. Using high-resolution melting analysis, we evaluated rs67085638 frequency in patients with the GC genotype (n = 214) and controls (n = 502) in a Polish Caucasian population. qRT-PCR was used to determine BMI1 transcripts. We observed the trend of rs67085638 association in all patients with GC ( p trend = 0.028), a strong risk of the GC genotype in male ( p trend = 0.035) but not female ( p trend = 0.747) patients, and the association with non-cardia GC ( p trend = 0.041), tumour stages T3 ( p trend = 0.014) and T4 ( p trend = 0.032), differentiation grading G3 ( p trend = 0.009), lymph node metastasis stage N3 ( p trend = 0.0005) and metastasis stage M0 ( p trend = 0.027). We found that significantly increased BMI1 transcripts were associated with the primary GC genotype classified as grade G3 ( p = 0.011) and as lymph node metastasis N3 ( p = 0.010) and counterpart marginal tissues ( p = 0.026, p = 0.040, respectively) from carriers of the T/T versus C/C genotypes. rs67085638 may contribute to increased BMI1 transcripts and the progression and rapid growth of GC.
Type of Medium:
Online Resource
ISSN:
2045-2322
DOI:
10.1038/s41598-021-94576-9
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2021
detail.hit.zdb_id:
2615211-3
