In:
Scientific Reports, Springer Science and Business Media LLC, Vol. 12, No. 1 ( 2022-01-20)
Abstract:
Sphingolipid long chain bases (LCBs) are building blocks of sphingolipids and can serve as signalling molecules, but also have antimicrobial activity and were effective in reducing growth of a range of human pathogens. In plants, LCBs are linked to cell death processes and the regulation of defence reactions against pathogens, but their role in directly influencing growth of plant-interacting microorganisms has received little attention. Therefore, we tested the major plant LCB phytosphingosine in in vitro tests with the plant pathogenic fungi Verticillium longisporum , Fusarium graminearum and Sclerotinia sclerotiorum , the plant symbiotic fungal endophyte Serendipita indica , the bacterial pathogens Pseudomonas syringae pv. tomato ( Pst ), Agrobacterium tumefaciens , and the related beneficial strain Rhizobium radiobacter . Phytosphingosine inhibited growth of these organisms at micromolar concentrations. Among the fungal pathogens, S. sclerotiorum was the most, and F. graminearum was the least sensitive. 15.9 μg/mL phytosphingosine effectively killed 95% of the three bacterial species. Plant disease symptoms and growth of Pst were also inhibited by phytosphingosine when co-infiltrated into Arabidopsis leaves, with no visible negative effect on host tissue. Taken together, we demonstrate that the plant LCB phytosphingosine inhibits growth of plant-interacting microorganisms. We discuss the potential of elevated LCB levels to enhance plant pathogen resistance.
Type of Medium:
Online Resource
ISSN:
2045-2322
DOI:
10.1038/s41598-022-05083-4
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2022
detail.hit.zdb_id:
2615211-3
