In:
Scientific Reports, Springer Science and Business Media LLC, Vol. 13, No. 1 ( 2023-09-26)
Abstract:
Although systolic function characteristically shows gradual impairment in pressure overload (PO)-evoked left ventricular (LV) hypertrophy (LVH), rapid progression to congestive heart failure (HF) occurs in distinct cases. The molecular mechanisms for the differences in maladaptation are unknown. Here, we examined microRNA (miRNA) expression and miRNA-driven posttranscriptional gene regulation in the two forms of PO-induced LVH (with/without systolic HF). PO was induced by aortic banding (AB) in male Sprague–Dawley rats. Sham-operated animals were controls. The majority of AB animals demonstrated concentric LVH and slightly decreased systolic function (termed as AB LVH ). In contrast, in some AB rats severely reduced ejection fraction, LV dilatation and increased lung weight-to-tibial length ratio was noted (referred to as AB HF ). Global LV miRNA sequencing revealed fifty differentially regulated miRNAs in AB HF compared to AB LVH . Network theoretical miRNA-target analysis predicted more than three thousand genes with miRNA-driven dysregulation between the two groups. Seventeen genes with high node strength value were selected for target validation, of which five ( Fmr1 , Zfpm2 , Wasl , Ets1, Atg16l1 ) showed decreased mRNA expression in AB HF by PCR. PO-evoked systolic HF is associated with unique miRNA alterations, which negatively regulate the mRNA expression of Fmr1 , Zfmp2 , Wasl , Ets1 and Atg16l1 .
Type of Medium:
Online Resource
ISSN:
2045-2322
DOI:
10.1038/s41598-023-43171-1
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2023
detail.hit.zdb_id:
2615211-3