In:
Scientific Reports, Springer Science and Business Media LLC, Vol. 5, No. 1 ( 2015-05-15)
Kurzfassung:
Cellular protrusions are highly dynamic structures involved in fundamental processes,including cell migration and invasion. For a cell to migrate, its leading edge mustform protrusions and then adhere or retract. The spatial and temporal coordinationof protrusions and retraction is yet to be fully understood. The study of protrusiondynamics mainly relies on live-microscopy often coupled to fluorescent labeling.Here we report the use of an alternative, label-free, quantitative and rapid assayto analyze protrusion dynamics in a cell population based on the real-time recordingof cell activity by means of electronic sensors. Cells are seeded on a plate coveredwith electrodes and their shape changes map into measured impedance variations. Upongrowth factor stimulation the impedance increases due to protrusive activity anddecreases following retraction. Compared to microscopy-based methods, impedancemeasurements are suitable to high-throughput studies on different cell lines, growthfactors and chemical compounds. We present data indicating that this assay lendsitself to dissect the biochemical signaling pathways controlling adhesiveprotrusions. Indeed, we show that the protrusion phase is sustained by actinpolymerization, directly driven by growth factor stimulation. Contraction insteadmainly relies on myosin action, pointing at a pivotal role of myosin in lamellipodiaretraction.
Materialart:
Online-Ressource
ISSN:
2045-2322
Sprache:
Englisch
Verlag:
Springer Science and Business Media LLC
Publikationsdatum:
2015
ZDB Id:
2615211-3
