In:
Scientific Reports, Springer Science and Business Media LLC, Vol. 6, No. 1 ( 2016-10-20)
Abstract:
The transcription factor Interferon Regulatory Factor 4 (IRF4) is essential for T H2 and T H17 cell formation and controls peripheral CD8 + T cell differentiation. We used Listeria monocytogenes infection to characterize the function of IRF4 in T H1 responses. IRF4 −/− mice generated only marginal numbers of listeria-specific T H1 cells. After transfer into infected mice, IRF4 −/− CD4 + T cells failed to differentiate into T H1 cells as indicated by reduced T-bet and IFN-γ expression, and showed limited proliferation. Activated IRF4 −/− CD4 + T cells exhibited diminished uptake of the glucose analog 2-NBDG, limited oxidative phosphorylation and strongly reduced aerobic glycolysis. Insufficient metabolic adaptation contributed to the limited proliferation and T H1 differentiation of IRF4 −/− CD4 + T cells. Our study identifies IRF4 as central regulator of T H1 responses and cellular metabolism. We propose that this function of IRF4 is fundamental for the initiation and maintenance of all T H cell responses.
Type of Medium:
Online Resource
ISSN:
2045-2322
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2016
detail.hit.zdb_id:
2615211-3