In:
RSC Advances, Royal Society of Chemistry (RSC), Vol. 13, No. 3 ( 2023), p. 1587-1593
Abstract:
A rapid, simple, and sensitive fluorescent detection method for brown spot of tobacco is established by lambda exonuclease-induced Mg 2+ -dependent DNAzyme amplification. It contains hybridization of the Alternaria alternata genome and HP1, digestion of the 5′-phosphorylated strand of the hybrid dsDNA by lambda exonuclease, acquisition of complete Mg 2+ -dependent DNAzyme, cleavage of the substrate modified with FAM and BHQ-1, and fluorescent detection. The proposed assay exhibits good sensitivity (10 pg L −1 ), selectivity and reproducibility. The method does not require pure DNA and expensive instruments, and can be performed within 2.5 hours. To the best of our knowledge, this is the first report of fluorescent detection of Alternaria alternata and its tobacco field samples. This method can be applied to the rapid and sensitive detection of Alternaria alternata in tobacco and its seedlings, and is particularly important for the green prevention and control of tobacco brown spot disease.
Type of Medium:
Online Resource
ISSN:
2046-2069
Language:
English
Publisher:
Royal Society of Chemistry (RSC)
Publication Date:
2023
detail.hit.zdb_id:
2623224-8
