In:
Biochemical Journal, Portland Press Ltd., Vol. 292, No. 3 ( 1993-06-15), p. 915-919
Abstract:
Rat liver mitochondrial acetyl-CoA acetyltransferase (acetoacetyl-CoA thiolase, EC 2.3.1.9) exists additionally in the CoA-modified forms A1 and A2. After a pulse of radioactivity using [35S]methionine in hepatocytes, the highest radioactivity was obtained in the unmodified enzyme. Over the chase time, the radioactivity in the unmodified enzyme decreased, but simultaneously increased in both CoA-modified forms, thus proving that the fully active unmodified enzyme exists before the partially active modified forms A1 and A2. Also, the specific radioactivity (ratio % radioactivity/% immunoreactive area) of A1 & gt; A2 demonstrates a sequential CoA modification of form A1 to form A2. Acetyl-CoA acetyltransferase was degraded with an apparent half-life of 38.0 h: the modified forms A1 and A2 have half-lives of 24.5 and 7.2 h. The physiological meaning of the CoA modification of acetyl-CoA acetyltransferase is not yet understood.
Type of Medium:
Online Resource
ISSN:
0264-6021
,
1470-8728
Language:
English
Publisher:
Portland Press Ltd.
Publication Date:
1993
detail.hit.zdb_id:
1473095-9
SSG:
12
