In:
British Journal of Haematology, Wiley, Vol. 92, No. 4 ( 1996-03), p. 788-794
Kurzfassung:
We studied the generation of human natural killer (NK) cells from CD34 + cells that were isolated from peripheral blood stem cells (PBSC) mobilized by granulocyte colony‐stimulating factor (G‐CSF). The isolated CD34 + cells were cultured in the presence of a combination of interleukin‐1 (IL‐1α), IL‐2, and stem cell factor for 5 weeks without marrow stroma. We found that the CD34 + cells isolated from G‐CSF‐mobilized PBSC (G‐CSF/PBSC) could differentiate into a population of NK cells which were CD56 +(bright) /CD3 − and showed morphologic characteristics of large granular lymphocytes. Immunophenotypic analysis of the NK cells thus generated showed that a small proportion of them expressed CD2, CD8 and CD16 surface markers and approximately half of them coexpressed CD7. This NK population exhibited cytotoxic activity against a NK‐sensitive cell line, K562. These observations suggest that CD34 + cells from G‐CSF/PBSC contain precursors of NK cells that can differentiate into functional NK cells.
Materialart:
Online-Ressource
ISSN:
0007-1048
,
1365-2141
DOI:
10.1046/j.1365-2141.1996.408950.x
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
1996
ZDB Id:
1475751-5
