In:
Applied Physics Letters, AIP Publishing, Vol. 66, No. 13 ( 1995-03-27), p. 1698-1700
Abstract:
The applicability of two photon excitation 4Pi confocal fluorescence microscopy to biological imaging is demonstrated. We show that 4Pi confocal microscopy in combination with a simple deconvolution algorithm allows axial localization and quantification with 0.14 μm resolution in a biological sample. The 4Pi-confocal microscope extends the applicability of far field fluorescence microscopy to high resolution three-dimensional imaging and quantification of subcellular structures.
Type of Medium:
Online Resource
ISSN:
0003-6951
,
1077-3118
Language:
English
Publisher:
AIP Publishing
Publication Date:
1995
detail.hit.zdb_id:
211245-0
detail.hit.zdb_id:
1469436-0