In:
Applied Physics Letters, AIP Publishing, Vol. 69, No. 4 ( 1996-07-22), p. 446-448
Abstract:
The change in position of fluorescent beads captured inside the focal volume of optical tweezers is monitored using fluorescence emission induced by two-photon absorption of a continuous-wave Nd:YAG laser (λ=1064 nm). The displacement of a bead due to interactions with its environment leads to a fluorescence intensity variation that is used to design a novel spatial sensor. We determine changes in the axial position of a CY3-labeled latex bead with a diameter of 1.03 μm to a precision better than 10 nm. At an intensity of 600 mW/ μm2 the two-photon bleaching rate is lower than 50% per 2000 s.
Type of Medium:
Online Resource
ISSN:
0003-6951
,
1077-3118
Language:
English
Publisher:
AIP Publishing
Publication Date:
1996
detail.hit.zdb_id:
211245-0
detail.hit.zdb_id:
1469436-0