In:
Applied Physics Letters, AIP Publishing, Vol. 89, No. 11 ( 2006-09-11)
Abstract:
The rupture force changes to separate the third strand from the duplex within an oligonucleotide-directed triplex (triplex DNA) were measured in the presence of small DNA binding molecules by means of atomic force spectroscopy. Rupture force histograms revealed a rupture force of 44.3±0.4pN to separate the third strand from duplex DNA in pure buffer solution at a rupture velocity of 400nm∕s. In 30μM of Ru(bipy)2(dppz)2+ buffer solution an increase of rupture force to 57±2.2pN was observed. For 30μM Ru(phen)2(dppz)2+ a rupture force of 65.1±3.9pN was determined. These results show that both intercalative molecules increase the stability of triplex DNA significantly.
Type of Medium:
Online Resource
ISSN:
0003-6951
,
1077-3118
Language:
English
Publisher:
AIP Publishing
Publication Date:
2006
detail.hit.zdb_id:
211245-0
detail.hit.zdb_id:
1469436-0