In:
Biomicrofluidics, AIP Publishing, Vol. 6, No. 3 ( 2012-09-01)
Abstract:
We present a straightforward microfluidics system to achieve step-by-step reaction sequences in a diffusion-controlled manner in quasi two-dimensional micro-confinements. We demonstrate the hierarchical self-organization of actin (actin monomers—entangled networks of filaments—networks of bundles) in a reversible fashion by tuning the Mg2+ ion concentration in the system. We show that actin can form networks of bundles in the presence of Mg2+ without any cross-linking proteins. The properties of these networks are influenced by the confinement geometry. In square microchambers we predominantly find rectangular networks, whereas triangular meshes are predominantly found in circular chambers.
Type of Medium:
Online Resource
ISSN:
1932-1058
Language:
English
Publisher:
AIP Publishing
Publication Date:
2012
detail.hit.zdb_id:
2265444-6
