In:
Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 97, No. 9 ( 2000-04-25), p. 4956-4960
Abstract:
An integrated molecular and physiological investigation of the
fundamental mechanisms of heavy metal accumulation was conducted in Thlaspi caerulescens , a Zn/Cd-hyperaccumulating plant
species. A heavy metal transporter cDNA, ZNT1 , was
cloned from T. caerulescens through functional
complementation in yeast and was shown to mediate high-affinity Zn 2+ uptake as well as low-affinity Cd 2+ uptake. It was found that this transporter is expressed at very high
levels in roots and shoots of the hyperaccumulator. A study of ZNT1 expression and high-affinity Zn 2+ uptake in roots of T. caerulescens and in a related
nonaccumulator, Thlaspi arvense , showed that alteration
in the regulation of ZNT1 gene expression by plant Zn
status results in the overexpression of this transporter and in increased Zn influx in roots of the hyperaccumulating Thlaspi species. These findings yield insights into the
molecular regulation and control of plant heavy metal and micronutrient accumulation and homeostasis, as well as provide information that will
contribute to the advancement of phytoremediation by the future engineering of plants with improved heavy metal uptake and tolerance.
Type of Medium:
Online Resource
ISSN:
0027-8424
,
1091-6490
DOI:
10.1073/pnas.97.9.4956
Language:
English
Publisher:
Proceedings of the National Academy of Sciences
Publication Date:
2000
detail.hit.zdb_id:
209104-5
detail.hit.zdb_id:
1461794-8
SSG:
11
SSG:
12
