In:
Molecular Biology of the Cell, American Society for Cell Biology (ASCB), Vol. 12, No. 9 ( 2001-09), p. 2835-2845
Abstract:
Calreticulin (CRT), a Ca 2+ -binding protein known to have many cellular functions, including regulation of Ca 2+ homoeostasis and chaperone activity, is essential for heart and brain development during embryogenesis in mice. Here, we report the functional characterization of Caenorhabditis elegans calreticulin (crt-1). Acrt-1 null mutant does not result in embryonic lethality but shows temperature-dependent reproduction defects. In C. elegans CRT-1 is expressed in the intestine, pharynx, body-wall muscles, head neurons, coelomocytes, and in sperm. crt-1males exhibit reduced mating efficiency and defects late in sperm development in addition to defects in oocyte development and/or somatic gonad function in hermaphrodites. Furthermore, crt-1 anditr-1 (inositol triphosphate receptor) together are required for normal behavioral rhythms. crt-1transcript level is elevated under stress conditions, suggesting that CRT-1 may be important for stress-induced chaperoning function inC. elegans.
Type of Medium:
Online Resource
ISSN:
1059-1524
,
1939-4586
DOI:
10.1091/mbc.12.9.2835
Language:
English
Publisher:
American Society for Cell Biology (ASCB)
Publication Date:
2001
detail.hit.zdb_id:
1474922-1
SSG:
12