In:
Molecular Biology of the Cell, American Society for Cell Biology (ASCB), Vol. 29, No. 8 ( 2018-04-15), p. 886-896
Kurzfassung:
Axonemal dyneins, including inner dynein arm I1, assemble in the cytoplasm prior to transport into cilia by intraflagellar transport (IFT). How I1 dynein interacts with IFT is not understood. We take advantage of the Chlamydomonas reinhardtii ida3 mutant, which assembles the inner arm I1 dynein complex in the cytoplasm but fails to transport I1 into the cilium, resulting in I1 dynein-deficient axonemes with abnormal motility. The IDA3 gene encodes an ∼115-kDa coiled-coil protein that primarily enters the cilium during ciliary growth but is not an axonemal protein. During growth, IDA3, along with I1 dynein, is transported by anterograde IFT to the tip of the cilium. At the tip, IDA3 uncouples from IFT and diffuses within the cilium. IFT transport of IDA3 decreases as cilia lengthen and subsides once full length is achieved. IDA3 is the first example of an essential and selective IFT adapter that is regulated by ciliary length.
Materialart:
Online-Ressource
ISSN:
1059-1524
,
1939-4586
DOI:
10.1091/mbc.E17-12-0729
Sprache:
Englisch
Verlag:
American Society for Cell Biology (ASCB)
Publikationsdatum:
2018
ZDB Id:
1474922-1
SSG:
12
