In:
Clinical Chemistry, Oxford University Press (OUP), Vol. 44, No. 2 ( 1998-02-01), p. 232-238
Kurzfassung:
Two peptide libraries, Ac-MXXXXXBBRM and Ac-VXXXXXBBRM, were constructed on TentaGel solid support to search for ligands that bind tightly with the H9724 Lyme antibody. By using an on-bead ELISA, approximately 120 ligands were selected as candidates for further study. Matrix-assisted laser desorption ionization mass spectrometry analysis of the candidate ligands indicated a high rate of occurrence of certain amino acids at the randomized positions. On the basis of the initial screening results, a small library was designed and iteratively synthesized. Subsequent library screenings led to the identification of four peptides, Ac-PQEEGX-NH2 (X = R, K, A, D), that showed specific affinity to the antibody. This combination of solid-phase screening and iterative synthesis is an effective strategy for rapid identification of ligands that bind tightly with disease-specific antibodies and should be applicable, at least in principle, to other ligand-receptor systems. This combinatorial library approach can also be a useful tool for the discovery of novel diagnostic agents.
Materialart:
Online-Ressource
ISSN:
0009-9147
,
1530-8561
DOI:
10.1093/clinchem/44.2.232
Sprache:
Englisch
Verlag:
Oxford University Press (OUP)
Publikationsdatum:
1998
