In:
Journal of AOAC INTERNATIONAL, Oxford University Press (OUP), Vol. 73, No. 5 ( 1990-09-01), p. 729-733
Abstract:
Twelve laboratories evaluated the Gram-Negative Identification (GNI) Card to Identify members of the Enterobacteriaceae. Eighty-four Isolates, previously Isolated from foods, were used In the collaborative study; the Isolates represented 12 genera within the Enterobacteriaceae group. Each collaborator streaked each isolate on tryptlc soy agar plates for purity. In the method, plates are Incubated 18-24 h at 35 °C. Isolated colonies are then subcultured to tryptlc soy agar slants and Incubated 18-24 h at 35 °C. An emulsion Is made from the growth on the slant In 1.8 mL 0.45% sodium chloride solution. The GNI Card Is filled and placed In a reader/Incubator. Isolates are Identified and an identification Is printed. The Vltek System correctly Identified 96.7% of Salmonella sp., 97.0 % of Escherichia coll, and an average of 93.8% of the other enteric genera. The method using the Vitek System and GNI Card has been approved interim official first action by AOAC as a screening method for the presumptive identification of Salmonella sp., E. coll, and other Enterobacteriaceae isolated from foods.
Type of Medium:
Online Resource
ISSN:
0004-5756
DOI:
10.1093/jaoac/73.5.729
Language:
English
Publisher:
Oxford University Press (OUP)
Publication Date:
1990