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    In: Journal of Animal Science, Oxford University Press (OUP), Vol. 98, No. Supplement_4 ( 2020-11-30), p. 442-443
    Abstract: Twelve crossbred steers fitted with rumen cannulae were used to determine the influence of trace mineral (TM) source on relative binding strength of minerals in the ruminal insoluble digesta fraction (IDF). Steers (n = 4/treatment) were fed a corn silage-based diet containing no supplemental Cu, Mn, and Zn for 7d. On d 8, steers received a bolus dose (2 x NRC requirement) of the respective TM from sulfate (STM), organic (ORG; provided as Zn methionine, Mn methionine, and Cu lysine), or hydroxy TM (HTM; Micronutrients USA LLC) sources. Ruminal samples were obtained at 2 h intervals starting at -4 and ending at 24 h post dosing. Ruminal samples were centrifuged and supernatant and the IDF were collected and frozen separately. Binding strength of Cu, Mn, and Zn to the IDF was estimated at 0, 12, and 24 h using dialysis against 0.05 M Tris-EDTA. At 0 h pre-dosing, IDF concentrations of Cu, Mn, and Zn were similar across treatments. At 12 h post dosing Cu and Zn IDF concentrations were greater (P & lt; 0.05) in HTM vs. STM and ORG supplemented steers, and Mn concentrations were greater (P & lt; 0.05) in HTM and ORG vs. STM supplemented steers. At 24 h post dosing, IDF Zn concentrations were greater (P & lt; 0.05) in HTM vs STM and ORG supplemented steers and Cu and Mn concentrations were similar across treatments. At 12 and 24 h post dosing the percent Cu and Zn released following dialysis was greater (P & lt; 0.05) in HTM vs STM and ORG supplemented steers. The percent released of Cu, Mn, and Zn at 0 h, and Mn at 12 and 24 h were similar across treatments. Data indicate that a greater concentration of Cu and Zn from HTM is loosely bound to the ruminal IDF.
    Type of Medium: Online Resource
    ISSN: 0021-8812 , 1525-3163
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2020
    detail.hit.zdb_id: 1490550-4
    SSG: 12
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