In:
Journal of Animal Science, Oxford University Press (OUP), Vol. 99, No. Supplement_3 ( 2021-10-08), p. 481-482
Abstract:
Six steers fitted with ruminal cannulae were used in a crossover design to examine the impact of direct fed microbial (DFM) supplementation on in vivo rumen fermentation characteristics and in vitro lactic acid clearance. Steers were fed a moderately high concentrate -1∙day-1 of 4.45x 1010 CFU/g of Propionibacteria acidipropionici). Treatments were administered daily, directly into the rumen via the cannula as a single bolus dose at the time of feeding. Rumen contents were sampled two h post-feeding daily and analyzed for short chain fatty acids (SCFA). On d 7 and 14, rumen fluid was collected from all steers and subjected to an in vitro lactic acid clearance challenge. Lactic acid and SCFA concentrations were determined at 3, 6 and 9 h post incubation. After d 14, all cattle received the basal diet for 21 d. On d 22, treatment crossover was implemented and the experiment repeated. Animal was considered the experimental unit and data were analyzed as a crossover design using Proc Mixed in SAS. In vivo propionic acid concentrations were greater (P & lt; 0.05) and total SCFA tended (P & lt; 0.06) to be greater in rumen fluid from steers receiving DFM. D- and total lactic acid concentrations were lesser (P & lt; 0.05) at 3 h post in vitro incubation for steers receiving DFM. D-, L+, and total lactic acid concentrations were similar between treatments at all other timepoints. In vitro propionic acid and total SCFA concentrations were greater (P & lt; 0.05) and acetic acid concentrations lesser (P & lt; 0.05) in steers receiving DFM. These data indicate that the DFM Propionibacteria acidipropionici, alters in vivo and in vitro rumen fermentation characteristics.
Type of Medium:
Online Resource
ISSN:
0021-8812
,
1525-3163
DOI:
10.1093/jas/skab235.849
Language:
English
Publisher:
Oxford University Press (OUP)
Publication Date:
2021
detail.hit.zdb_id:
1490550-4
SSG:
12
