In:
Journal of Industrial Microbiology and Biotechnology, Oxford University Press (OUP), ( 2023-11-13)
Abstract:
Gas chromatography-tandem mass spectrometry with electron ionization (GC-EI-MS/MS) provides rich information on stable-isotope labeling for 13C-metabolic flux analysis (13C-MFA). To pave the way for the routine application of tandem MS data for metabolic flux quantification, we aimed to compile a comprehensive library of GC-EI-MS/MS fragments of tert-butyldimethylsilyl (TBDMS) derivatized proteinogenic amino acids. First, we established an analytical workflow which combines high resolution gas chromatography-quadrupole time-of-flight mass spectrometry (GC-EI-QTOFMS) and fully 13C-labeled biomass to identify and structurally elucidate tandem MS amino acid fragments. Application of the high-mass accuracy MS procedure resulted into the identification of 129 validated precursor-product ion pairs of 13 amino acids with 30 fragments being accepted for 13C-MFA. The practical benefit of the novel tandem MS data was demonstrated by a proof-of-concept study which confirmed the importance of the compiled library for high resolution 13C-MFA.
Type of Medium:
Online Resource
ISSN:
1367-5435
,
1476-5535
DOI:
10.1093/jimb/kuad039
Language:
English
Publisher:
Oxford University Press (OUP)
Publication Date:
2023
detail.hit.zdb_id:
1482484-X
SSG:
12