In:
Plant Disease, Scientific Societies
Abstract:
Xanthomonas fragariae (X. fragariae) is classified as a quarantine pathogen by the European and Mediterranean Plant Protection Organization. It commonly induces typical angular leaf spot (ALS) symptoms in strawberry leaves. X. fragariae strains from China (YL19, SHAQP01, and YLX21) exhibit ALS symptoms in leaves and more severe symptoms of dry cavity rot in strawberry crowns. Conversely, strains from other countries do not cause severe dry cavity rot symptoms in strawberries. Employing multilocus sequence analysis (MLSA), average nucleotide identity (ANI), and amino acid identity (AAI), we determined that Chinese strains of X. fragariae are genetically distinct from other strains and can be considered a new subspecies. Subsequent analysis of 63 X. fragariae genomes published at NCBI using IPGA and EDGAR3.0 revealed the pan-genomic profile, with 1680 shared genes present in the all 63 strains, including 71 virulence-related genes. Additionally, we identified 123 genes exclusive to the of all Chinese strains, encompassing 12 virulence-related genes. The qRT-PCR analysis demonstrated that the expression of XopD, XopG1, CE8, GT2 and GH121, out of 12 virulence-related genes of Chinese strains (YL19) exhibited a constant increase in the early stages (6 hpi, 24 hpi, 54 hpi, and 96 hpi) of strawberry leaf infected by YL19. Hence, the presence of XopD, XopG1, CE8, GT2, and GH121 in Chinese strains may play important roles in the early infection process of Chinese strains. These findings offer novel insights into comprehending the population structure and variation in the pathogenic capacity of X. fragariae.
Type of Medium:
Online Resource
ISSN:
0191-2917
,
1943-7692
DOI:
10.1094/PDIS-05-23-0933-SC
Language:
English
Publisher:
Scientific Societies
Publication Date:
2023
detail.hit.zdb_id:
2042679-3
