In:
The FASEB Journal, Wiley, Vol. 37, No. 9 ( 2023-09)
Kurzfassung:
Recent advances in gene therapy have brought novel treatment options for cancer. However, the full potential of this approach has yet to be unlocked due to the limited payload capacity of commonly utilized viral vectors. Virus‐free DNA transposons, including piggyBac , have the potential to obviate these shortcomings. In this study, we improved a previously modified piggyBac system with superior transposition efficiency. We demonstrated that the internal domain sequences (IDS) within the 3′ terminal repeat domain of hyperactive piggyBac ( hyPB ) donor vector contain dominant enhancer elements. Plasmid‐free donor vector devoid of IDS was used in conjunction with a helper plasmid expressing Quantum PBase ™ v2 to generate an optimal piggyBac system, Quantum pBac ™ ( qPB ), for use in T cells. qPB outperformed hyPB in CD20/CD19 CAR‐T production in terms of performance as well as yield of the CAR‐T cells produced. Furthermore, qPB also produced CAR‐T cells with lower donor‐associated variabilities compared to lentiviral vector. Importantly, qPB yielded mainly CD8 + CAR‐T SCM cells, and the qPB‐ produced CAR‐T cells effectively eliminated CD20/CD19‐expressing tumor cells both in vitro and in vivo. Our findings confirm qPB as a promising virus‐free vector system with an enhanced payload capacity to incorporate multiple genes. This highly efficient and potentially safe system will be expected to further advance gene therapy applications.
Materialart:
Online-Ressource
ISSN:
0892-6638
,
1530-6860
DOI:
10.1096/fj.202201654R
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2023
ZDB Id:
1468876-1
SSG:
12
