In:
Critical Care Medicine, Ovid Technologies (Wolters Kluwer Health), Vol. 44, No. 11 ( 2016-11), p. e1132-e1136
Kurzfassung:
To investigate the contribution of neutrophil activation as innate immune cells during septic shock–induced disseminated intravascular coagulation. Design: Prospective study. Setting: One University Hospital ICU. Participants: Hundred patients with septic shock. Thirty-five patients had disseminated intravascular coagulation according to Japanese Association for Acute Medicine 2006 score. Intervention: None. Measurements and Main Results: Neutrophil chromatin decondensation was assessed by measuring neutrophil fluorescence (NEUT-side-fluorescence light) labeled by a fluorochrome-based polymethine reagent using a routine automated flow cytometer Sysmex XN20 (Sysmex, Kobe, Japan) and neutrophil-derived CD66b microparticles by prothrombinase assay. Measurements in disseminated intravascular coagulation and no disseminated intravascular coagulation patients showed that a mean value of NEUT-side-fluorescence light above 57.3 arbitrary units had a sensitivity of 90.91% and a specificity of 80.60% for disseminated intravascular coagulation diagnosis. NEUT-side-fluorescence light was correlated to the CD66b microparticles/neutrophil count, a surrogate of neutrophil activation associated with septic shock–induced disseminated intravascular coagulation. Conclusion: NEUT-side-fluorescence light, routinely available, could prove an accurate biomarker of neutrophil activation.
Materialart:
Online-Ressource
ISSN:
0090-3493
DOI:
10.1097/CCM.0000000000001851
Sprache:
Englisch
Verlag:
Ovid Technologies (Wolters Kluwer Health)
Publikationsdatum:
2016
ZDB Id:
2034247-0