In:
Hepatology, Ovid Technologies (Wolters Kluwer Health)
Abstract:
Genetic risk factors are major determinants of chronic liver disease (CDL) progression. Patatin-like phospholipase domain-containing protein 3 (PNPLA3) I148M polymorphism and alpha-1 antitrypsin E342K variant, termed PiZ, are major modifiers of metabolic CLD. Both variants are known to affect metabolic CLD via increased endoplasmic reticulum stress, but their combined effect on CLD progression remains largely unknown. Here we aimed to test our working hypothesis that their combined incidence triggers CLD disease progression. Approach and Results: We showed that PiZZ/PNPLA3 I148M patients from the European AATD liver consortium and UK Biobank had a trend towards higher liver enzymes, but no increased liver fat accumulation was evident between subgroups. After generating transgenic mice that overexpress the PiZ variant and simultaneously harbor the PNPLA3 I148M knockin (designated as PiZ/PNPLA3 I148M ), we observed that PiZ and PiZ/PNPLA3 I148M animals showed increased liver enzymes compared to controls during aging. However, no significant difference between PiZ and PiZ/PNPLA3 I148M groups was observed with no increased liver fat accumulation over time. To further study the impact on CLD progression a Western-Styled diet (WSD) was administered, which resulted in increased fat accumulation and fibrosis in PiZ and PiZ/PNPLA3 I148M livers compared to controls, but the additional presence of PNPLA3 I148M had no impact on liver phenotype. Notably, PiZ variant protected PNPLA3 I148M mice from liver damage and obesity after WSD feeding. Conclusion: Our results demonstrate that the PNPLA3 polymorphism in the absence of additional metabolic risk factors is insufficient to drive the development of advanced liver disease in severe alpha1-antitrypsin deficiency.
Type of Medium:
Online Resource
ISSN:
0270-9139
DOI:
10.1097/HEP.0000000000000574
Language:
English
Publisher:
Ovid Technologies (Wolters Kluwer Health)
Publication Date:
2023
detail.hit.zdb_id:
1472120-X
