In:
Otology & Neurotology, Ovid Technologies (Wolters Kluwer Health), Vol. 40, No. 2 ( 2019-02), p. e99-e106
Kurzfassung:
This prospective study aimed to evaluate the diagnostic and clinical utility of the myeloperoxidase (MPO)–DNA complex as a NETosis-derived product in the middle ear fluid of patients with otitis media with antineutrophil cytoplasmic antibody-associated vasculitis (OMAAV). Study Design: Prospective study. Setting: Tertiary referral center. Patients: Twenty-two patients diagnosed with OMAAV. Intervention: Collection of the fluid samples from middle ear. Main Outcome Measure: The levels of the MPO–DNA complex in the fluid samples were quantified using an enzyme-linked immunosorbent assay. Results: Patients with both systemic and localized forms of OMAAV showed significantly higher levels of the MPO–DNA complex compared to the controls ( p 〈 0.001 and p = 0.002, respectively). In particular, they showed significantly higher levels of MPO–DNA complex compared to the controls, regardless of serum antineutrophil cytoplasmic antibody status ( p 〈 0.001 and p 〈 0.001, respectively) or immunosuppressive therapy ( p 〈 0.001 and p 〈 0.001, respectively) at the time of sampling. An optical density cutoff value of 0.16 at 405 nm according to the receiver operating characteristic curve showed a sensitivity of 86.4%, specificity of 95.5%, positive predictive value of 95.0% and negative predictive value of 87.5% for the diagnosis of OMAAV. Significant positive correlations were observed between the levels of MPO–DNA complex and the values for air conduction – ( r = 0.49, p = 0.022) and bone conduction – pure tone average thresholds ( r = 0.45, p = 0.035). Conclusions: The detection and quantification of the MPO–DNA complex in the otitis media fluid may aid in providing a definite diagnosis as well as predicting the activity and severity of OMAAV.
Materialart:
Online-Ressource
ISSN:
1531-7129
,
1537-4505
DOI:
10.1097/MAO.0000000000002081
Sprache:
Englisch
Verlag:
Ovid Technologies (Wolters Kluwer Health)
Publikationsdatum:
2019
ZDB Id:
2058738-7
