In:
Pancreas, Ovid Technologies (Wolters Kluwer Health), Vol. 50, No. 8 ( 2021-9), p. 1202-1211
Kurzfassung:
The intestinal barrier injury caused by severe acute pancreatitis (SAP) can induce enterogenous infection, further aggravating the inflammatory reactions and immune responses. This study aimed to test the hypothesis that emodin protects the intestinal function and is involved in the immune response in SAP. Methods The network pharmacology was established using the Swiss target prediction and pathway enrichment analysis. The SAP mice model was induced by cerulein (50 μg/kg) and lipopolysaccharide (10 mg/kg) hyperstimulation. The pharmacological effect of emodin in treating SAP was evaluated at mRNA and protein levels by various methods. Results The network analysis provided the connectivity between the targets of emodin and the intestinal barrier–associated proteins and predicted the BAX/Bcl-2/caspase 3 signaling pathway. Emodin alleviated the pathological damages to the pancreas and intestine and reduced the high concentrations of serum amylase and cytokines in vivo. Emodin increased the expression of intestinal barrier–related proteins and reversed the changes in the apoptosis-related proteins in the intestine. Simultaneously, emodin regulated the ratio of T helper type 1 (T H 1), T H 2, T H 17, γδ T cells, and interferon γ/interleukin 17 producing γδ T cells. Conclusions These findings partly verified the mechanism underlying the regulation of the intestinal barrier and immune response by emodin.
Materialart:
Online-Ressource
ISSN:
1536-4828
,
0885-3177
DOI:
10.1097/MPA.0000000000001894
Sprache:
Englisch
Verlag:
Ovid Technologies (Wolters Kluwer Health)
Publikationsdatum:
2021
ZDB Id:
2053902-2
