In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 65, No. Pt_5 ( 2015-05-01), p. 1444-1449
Abstract:
Five non-spore-forming, aerobic and Gram-stain-positive bacterial strains, 10-107-8 T , 1C-4, NHI3_6, 4107_1_2, and 3D-3, were isolated from Populus × euramericana bark collected in Puyang City, Henan Province, PR China. The isolates grew at 15–40 °C and pH 5–10. The optimum temperature and pH for growth were 30 °C and pH 8.0, respectively. Chemotaxonomic features included MK-10 and MK-11 as major menaquinones (type strain); predominating iso- and anteiso-branched cellular fatty acids; diphosphatidylglycerol and phosphatidylglycerol as major polar lipids (type strain); ornithine as the principal diamino acid of the cell-wall peptidoglycan (type strain); glycolyl type as cell-wall acyl type; and DNA G+C content of 66.8–67.6 mol%. These features were consistent with classification in the genus Microbacterium . Analysis of 16S rRNA gene sequence data indicated that the five isolates belonged to the genus Microbacterium and were closely related to Microbacterium halotolerans . A high 16S rRNA gene sequence similarity of 96.97 % to M. halotolerans YIM 70130 T was observed. The five isolates showed less than 96.20 % 16S rRNA gene sequence similarity to the other species of the genus Microbacterium with validly published names. DNA–DNA relatedness of the five isolates with M. halotolerans JCM 13013 T ranged from 35.62 % to 44.36 %. Considering the results of 16S rRNA gene sequence analysis and the physiological and biochemical characteristics, we propose that the five strains should be assigned to a novel species of the genus Microbacterium . The name proposed for the five strains is Microbacterium populi sp. nov., and the type strain is 10-107-8 T ( = CFCC 11275 T = KCTC 29152 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.000117
Language:
English
Publisher:
Microbiology Society
Publication Date:
2015
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
