In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 60, No. 3 ( 2010-03-01), p. 515-525
Abstract:
Three pathogenic variants (i.e. pathotypes) have been described within Xanthomonas citri pv. citri , the causal agent of Asiatic citrus canker. Pathotype A strains naturally infect a wide range of Citrus species and members of some related genera. In contrast, pathotypes A* and A w have narrow host ranges within the genus Citrus and have been isolated from Mexican lime ( Citrus aurantifolia L.) and from Mexican lime and alemow ( Citrus macrophylla L.), respectively. We used amplified fragment length polymorphism (AFLP) and multilocus sequence analysis (MLSA) based on four partial housekeeping gene sequences ( atpD , dnaK , efp and gyrB ) for the genotypic classification of Xanthomonas citri pv. citri and the poorly characterized citrus pathogen Xanthomonas campestris pv. bilvae . A Mantel test showed that genetic distances derived from AFLP and MLSA were highly correlated. X. campestris pv. bilvae showed a close relatedness to the type strain of X. citri , indicating that this pathovar should be reclassified as X. citri pv. bilvae . All pathotype A* and A w strains were most closely related to X. citri pv. citri strains with a wide host range (pathotype A), confirming previous DNA–DNA hybridization data. Pathotype A w should be considered a junior synonym of pathotype A* on the basis of pathogenicity tests, AFLP, MLSA and PCR using pathovar-specific primers. Evolutionary genome divergences computed from AFLP data suggested that pathotype A* (including A w strains) is a group of strains that shows a wider genetic diversity than pathotype A.
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.009514-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2010
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
