In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 60, No. 11 ( 2010-11-01), p. 2601-2605
Abstract:
A novel gammaproteobacterium, strain CN3 T , was isolated from the faeces of wild boar. Strain CN3 T was facultatively anaerobic and appeared coccoid or rod-shaped. The partial 16S rRNA gene sequence determined for strain CN3 T suggested a distant relationship with members of the orders ‘ Enterobacteriales ’ and Pasteurellales . The gene sequence showed highest similarity (90.3 %) with Obesumbacterium proteus DSM 2777 T , a member of the family Enterobacteriaceae . The closest relatives outside the order ‘ Enterobacteriales ’ according to 16S rRNA gene sequence analysis were members of the order Pasteurellales with 88.7 % similarity ( Mannheimia haemolytica NCTC 9380 T and Actinobacillus lignieresii NCTC 4189 T ). In contrast to most members of the order ‘ Enterobacteriales ’, strain CN3 T was oxidase-positive. The pattern of fatty acids, in particular the high relative abundance of C 18 : 1 ω 7 c (38.5 %), was clearly distinct from the conserved pattern found for members of the order Pasteurellales . Eco RI ribotyping of strain CN3 T yielded no significant similarity to existing database entries. The major ubiquinone of strain CN3 T was Q-8. The DNA G+C content was 36.4 mol%. Strain CN3 T hosted a phage and secreted considerable amounts of three proteins into the culture supernatant. A spontaneous mutant of strain CN3 T was isolated which formed long filaments. Microscopic studies revealed the presence of a capsule that the mutant strain was unable to partition after cell division. Strain CN3 T thus represents a novel species within a new genus, for which the name Orbus hercynius gen. nov., sp. nov. is proposed. The type strain of the type species is CN3 T (=DSM 22228 T =CCUG 57622 T ). Classification of the novel species to the family and order level will require further investigations.
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.019026-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2010
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
