In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 62, No. Pt_7 ( 2012-07-01), p. 1576-1580
Kurzfassung:
A novel bacterium (strain YIM 65583 T ) belonging to the genus Sphingomonas was isolated from surface-sterilized tissue of Artemisia annua L., which was collected from Yunnan province, south-west China. Its morphology, physiology and biochemical features were consistent with those of members of the genus Sphingomonas . Analysis of the 16S rRNA gene sequence of strain YIM 65583 T further confirmed that it should be classified as a member of the genus Sphingomonas and was most closely related to Sphingomonas phyllosphaerae FA2 T (99.7 %) and Sphingomonas yunnanensis YIM 003 T (98.3 %). The isolate was Gram-negative and formed yellow-pigmented colonies on ISP 2 medium. It grew optimally at pH 6.0–8.0, at 20–37 °C and in the presence of 0–1 % (w/v) NaCl. The major respiratory lipoquinone was ubiquinone-10; C 18 : 1 ω7 c , anteiso-C 16 : 1 , C 14 : 0 -2OH, C 17 : 1 ω6 c , C 16 : 0 and C 15 : 0 were the major fatty acids. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and sphingoglycolipid. The G+C content of the genomic DNA was 63.3 mol%. The DNA–DNA relatedness values of the isolate YIM 65583 T with S. phyllosphaerae FA2 T and S. yunnanensis YIM 003 T were 43.1 % and 37.9 %, respectively. Based on these features, it is concluded that the strain represents a novel species of the genus Sphingomona s, for which the name Sphingomonas endophytica sp. nov. is proposed, with YIM 65583 T ( = CCTCC AA 209035 T = JCM 17394 T ) as the type strain.
Materialart:
Online-Ressource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.031484-0
Sprache:
Englisch
Verlag:
Microbiology Society
Publikationsdatum:
2012
ZDB Id:
215062-1
ZDB Id:
2056611-6
SSG:
12
